Utilization of Palm Oil Mill Effluent (POME) from the Residual Biogas Power Plant for Microalgae Production as Raw Material of Biodiesel

  • Rahmat Wahyudi Nasution
  • Erliza Hambali
  • Suprihatin
Keywords: POME, Cultivated Microalgae, Lipid Extraction, Osmotic Shock


Palm Oil Mill Effluent (POME) from the residue of biogas power plants generated by the Palm Oil Mill as the waste is still a problem in the processing environment and the environmental impact. However, POME waste still contains nutrients which are essential to the growth of microalgae as a growing medium. Utilization of POME can improve the growth of microalgae Botryococcus braunii compared to conventional media. By doing this project, the cost used by the palm oil mill waste management can be reduced and even be able to provide profits for palm oil mill and also can reduce the negative impact on the environment. The study was conducted at the Microalgae Laboratory of SBRC - IPB in September-Oktober 2018. Botryococcus braunii was cultivated in 1,000 ml erlenmeyer compared to standard medium as the control solution of 0.1% POME from the residue biogas power plants. Observation of Botryococcus braunii growth curve has carried out in accordance. Studies were arranged in CRD, with three replication. The results showed that the rate of Botryococcus braunii growth in POME is higher than the control for 2.7x106 sel/ml. And lipid extraction using osmotic shock method with HCl and CH3COONa as osmotic agents in concentrations of 0.1; 0.3; 0.5 M, the highest yield of 0.108 g was obtained on the osmotic agent CH3COONa 0.3 M.


[1] Kawaroe. (2010). Mikroalga potensi dan pemanfaatannya untuk produksi bio bahan bakar. Bogor: IPB Press.
[2] Mercer, P. & Armenta, R.E. (2011). Developments in oil extraction from microalgae. European Journal of Lipid Science and Technology, 113, 539–547.
[3] Rachmaniah ORD & Setyarini L, Maulida. (2010). Pemilihan metode ekstraksi minyakalga dari chlorella sp. dan prediksinya sebagai biodiesel. Seminar Teknik Kimia Soehadi Reksowardojo, Surabaya.
[4] Yoo G, Parka WK, Kima CW, Choi YE, & Yanga JW. (2012). Direct lipid extraction from wet chlamydomonas reinhardtii biomass using osmotic shock. Bioresource Technology, 123, 717-722.
[5] Junaidi AB, Zulfikurrahman, Abdullah, & Gunawan. (2014). Ekstraksi lipid dari biomassa synechococcus sp. dengan metode osmotic shock. Jurnal Sains dan Terapan Kimia, 8(2), 94-102.
[6] Hendroko R, Kawaroe M, Salafudin, Ganjar S, Nur EF, Dahlia WS, Yosepinus S. (2011). Biorefinery Preliminary: Integration of slurry and CO2 as biomethane digeste waste for microalgaescenedesmus sp. growth. International Seminar on Chemical Enginering, Soehardi Reksowardojo.
[7] Kawaroe M., Hwangbo J., Augustine D., & Putra H.A. (2015). Comparison of density, specific growth rate, biomass weight, and doubling time of micro algaenannochloropsis sp. cultivated in open raceway pond and photobioreactor. Available at: http://www.bioflux.com.ro/docs/2015.740-750.pdf.
[8] Sandra Hermanto, Anna Muawanah, & Rizkina Harahap. (2008). Profil dan karakteristik lemak hewani (Ayam, Sapi dan Babi) hasil analisa FTIR dan GCMS. Available at: https://pdfs.semanticscholar.org/5233/fe12ede6a18e7e5e6a99c103def1f47c5820.pdf.
[9] Dunlap, C.A., K.O. Evans, B. Theelen, T. Boekhout, & D. Schisler. (2007). Osmotic shock tolerance and membrane fluidity of cold adapted cryptococcus flavescens OH182.9. Available at: http://www.wi.knaw.nl/images/ResearchGroups/Publications/2007Dunlap0001.pdf.
[10] Christie WW. (2012). Mass spectra of methyl esters of fatty acids. Part 1. Normal Saturated Fatty Acids.
[11] Borowitzka MA. (1988). Micro-algal biotechnology. New York: Cambridge University Press.
[12] Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M., & Darzins A. (2008). Microalgal triacylglycerols as feedstocks for biofuel productions: Perspectives and advances. Available at: https://www.ncbi.nlm.nih.gov/pubmed/18476868.
[13] Mittelbach M & Remschmidt C. (2006). Biodiesel: The comprehensive handbook. (3rd ed.). Grez: Boersedruck Ges.
How to Cite
Rahmat Wahyudi Nasution, Erliza Hambali, & Suprihatin. (2019). Utilization of Palm Oil Mill Effluent (POME) from the Residual Biogas Power Plant for Microalgae Production as Raw Material of Biodiesel. International Journal of Engineering and Management Research, 9(5), 141-145. Retrieved from http://www.ijemr.net/ojs/index.php/ojs/article/view/47